概述

  • 产品名称Anti-HGS抗体
    参阅全部 HGS 一抗
  • 描述
    山羊多克隆抗体to HGS
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Xenopus laevis, Pufferfish (Fugu), Zebrafish
  • 免疫原

    Synthetic peptide:

    NRNYWEKKQEEARK

    , corresponding to internal sequence amino acids 331-344 of Human HGS.

  • 阳性对照
    • Lysates of Human monocytic leukemia cell line U937.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab48014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 组织特异性Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • 序列相似性Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 结构域Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 翻译后修饰Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • 细胞定位Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Anti-HGS antibody 图像

  • ab48014  (2.5 µg/ml) staining HGS in Human Testis by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows strong staining of Leydig cells.

Anti-HGS antibody (ab48014)参考文献

ab48014 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab48014.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"