概述

性能

应用

Our Abpromise guarantee covers the use of ab83760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21121876
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with HGF peptide (ab189221). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration. ELISA titre using peptide based assay 1/312500.

靶标

Anti-HGF antibody 图像

  • Anti-HGF antibody (ab83760) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Gel concentration 12%
  • ab83760 staining HGF in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

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  • ICC/IF image of ab83760 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83760, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry of Human Adrenal Gland tissue at an antibody concentration of 5.0µg/ml using anti-HGF antibody (ab83760)

Anti-HGF antibody (ab83760)参考文献

This product has been referenced in:
  • Zhang X  et al. Hypoxia-inducible factor-1a mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions. Oncol Lett 12:1034-1040 (2016). WB ; Human . Read more (PubMed: 27446390) »
  • Hamilton A  et al. Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation. PLoS One 10:e0126688 (2015). Mouse . Read more (PubMed: 25978773) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 11 2016

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

Abreviews
Application Western blot
Loading amount 60 µg
Gel Running Conditions Non-reduced Denaturing (gel 10%)
Sample Dog Cell lysate - whole cell (adipose tissue derived mesencymal stem cell)
Specification adipose tissue derived mesencymal stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Dr. SeungHoon Lee

Verified customer

提交于 Sep 10 2013

Thank you for contacting us today.
As requested, we have now uploaded the materials datasheet onto the datasheet of the Anti-HGF antibody (ab83760). This should be available online in the next few hours.
I have also attached a copy to this emai...

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Thank you for contacting us.
For HGF (ab83760), I would recommend 293T whole cell lysate - ab95494. For CTGF (ab6992), I would recommend human hippocampal whole cell lysate - ab30180. I hope this helps, please let me know if you need any additiona...

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DISCOUNT CODE: xxxxx Expiration date: xxxxx I am very pleased to hear your customer would like to accept our offer and test ab83760 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please s...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"