概述

性能

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应用

Our Abpromise guarantee covers the use of ab24865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. (see Abreview)
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab10829 as Capture.

靶标

  • 功能Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 疾病相关Deafness, autosomal recessive, 39
  • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DFNB39 antibody
    • F TCF antibody
    • Fibroblast derived tumor cytotoxic factor antibody
    • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
    • Hepatocyte growth factor antibody
    • Hepatocyte growth factor beta chain antibody
    • Hepatocyte growth factor precursor antibody
    • Hepatopoietin A antibody
    • Hepatopoietin-A antibody
    • Hgf antibody
    • HGF_HUMAN antibody
    • HGFB antibody
    • HPTA antibody
    • Lung fibroblast derived mitogen antibody
    • OTTHUMP00000161349 antibody
    • OTTHUMP00000206710 antibody
    • OTTHUMP00000206711 antibody
    • OTTHUMP00000206712 antibody
    • OTTHUMP00000206713 antibody
    • OTTHUMP00000206730 antibody
    • Scatter factor antibody
    • SF antibody
    see all

Anti-HGF antibody 图像

  • Standard Curve for HGF (Analyte: ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.
  • Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-HGF antibody (ab24865)参考文献

This product has been referenced in:
  • Cohen ME  et al. Signaling through three chemokine receptors triggers the migration of transplanted neural precursor cells in a model of multiple sclerosis. Stem Cell Res 13:227-39 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25086214) »
  • Goldoni S  et al. Decorin is a novel antagonistic ligand of the Met receptor. J Cell Biol 185:743-54 (2009). WB ; Human . Read more (PubMed: 19433454) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

The antibody was generated against the active recombinant protein of HGF, so it should also recognize the inactive precursor form. However we do not have the possibility to check it.

Also, there is a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human mesenchymal stem cells transduced with Adv-H)
Specification Human mesenchymal stem cells transduced with Adv-H
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Mr. Hao Wu

Verified customer

提交于 Dec 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"