Customer reviews and FAQs

概述

性能

应用

Our Abpromise guarantee covers the use of ab24865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. (see Abreview)
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab10829 as Capture.

靶标

  • 功能
    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 疾病相关
    Deafness, autosomal recessive, 39
  • 序列相似性
    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DFNB39 antibody
      • F TCF antibody
      • Fibroblast derived tumor cytotoxic factor antibody
      • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
      • Hepatocyte growth factor antibody
      • Hepatocyte growth factor beta chain antibody
      • Hepatocyte growth factor precursor antibody
      • Hepatopoietin A antibody
      • Hepatopoietin-A antibody
      • Hgf antibody
      • HGF_HUMAN antibody
      • HGFB antibody
      • HPTA antibody
      • Lung fibroblast derived mitogen antibody
      • OTTHUMP00000161349 antibody
      • OTTHUMP00000206710 antibody
      • OTTHUMP00000206711 antibody
      • OTTHUMP00000206712 antibody
      • OTTHUMP00000206713 antibody
      • OTTHUMP00000206730 antibody
      • Scatter factor antibody
      • SF antibody
      see all

    Anti-HGF antibody 图像

    • Sandwich ELISA - HGF antibody (ab24865)
      Sandwich ELISA - HGF antibody (ab24865)
      Standard Curve for HGF (Analyte: ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.
    • Western blot
      Western blot
      Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    Anti-HGF antibody (ab24865)参考文献

    This product has been referenced in:
    • Cohen ME  et al. Signaling through three chemokine receptors triggers the migration of transplanted neural precursor cells in a model of multiple sclerosis. Stem Cell Res 13:227-39 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25086214) »
    • Goldoni S  et al. Decorin is a novel antagonistic ligand of the Met receptor. J Cell Biol 185:743-54 (2009). WB ; Human . Read more (PubMed: 19433454) »

    See all 2 Publications for this product

    发表研究结果有使用 ab24865？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab24865 的 abreview 提交 ab24865 的问题

    3 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HGF antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil,cerebellum,fetal liver,normal breast,fetal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
    Permeabilization
    Yes - 0.05%Tween20 in PBS
    Specification
    Tonsil,cerebellum,fetal liver,normal breast,fetal
    Blocking step
    sea block (fisher#37527) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Apr 28 2017

    Will this antibody recognize the inactive & active form of the protein?

    Thank you for contacting Abcam.

    The antibody was generated against the active recombinant protein of HGF, so it should also recognize the inactive precursor form. However we do not have the possibility to check it.

    Also, there is a...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HGF antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human mesenchymal stem cells transduced with Adv-H)
    Specification
    Human mesenchymal stem cells transduced with Adv-H
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    abreview image
    Username

    Mr. Hao Wu

    Verified customer

    提交于 Dec 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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