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Our Abpromise guarantee covers the use of ab24865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. (see Abreview)|
|ELISA||Use a concentration of 1 - 15 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab10829 as Capture.|