概述

  • 产品名称
  • 描述
    兔多克隆抗体to HEY2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse HEY2/ Hry2.

    (Peptide available as ab38053.)

  • 阳性对照
    • ab25404 gave a positive signal in Mouse Heart and Skeletal Muscle Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab25404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能
    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • 序列相似性
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHb32 antibody
    • Cardiovascular helix loop helix factor 1 antibody
    • Cardiovascular helix-loop-helix factor 1 antibody
    • CHF1 antibody
    • Class B basic helix-loop-helix protein 32 antibody
    • GRIDLOCK antibody
    • GRL antibody
    • Hairy and enhancer of split related 2 antibody
    • Hairy and enhancer of split-related protein 2 antibody
    • Hairy related transcription factor 2 antibody
    • Hairy-related transcription factor 2 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
    • hCHF1 antibody
    • HERP antibody
    • HERP1 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-2 antibody
    • HESR2 antibody
    • hey2 antibody
    • HEY2_HUMAN antibody
    • hHRT2 antibody
    • HRT-2 antibody
    • HRT2 antibody
    • MGC10720 antibody
    • Protein gridlock homolog antibody
    see all

Anti-HEY2 antibody 图像

  • All lanes : Anti-HEY2 antibody (ab25404) at 2 µg/ml

    Lane 1 : Heart (Mouse)Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-HEY2 antibody (ab25404)参考文献

This product has been referenced in:
  • Redmond EM  et al. Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling. PLoS One 9:e84122 (2014). WB ; Mouse . Read more (PubMed: 24416200) »
  • Hu GH  et al. Delta-like ligand 4 (Dll4) predicts the prognosis of clear cell renal cell carcinoma, and anti-Dll4 suppresses tumor growth in vivo. Int J Clin Exp Pathol 7:2143-52 (2014). WB . Read more (PubMed: 24966922) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEY2 overexpressing human pluripotent stem cell)
Permeabilization
Yes - saponin
Specification
HEY2 overexpressing human pluripotent stem cell
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 12 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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