概述

  • 产品名称
  • 描述
    兔多克隆抗体to HEY1
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide derived from a region between residues 207-261 of Human HEY1.

  • 阳性对照
    • Jurkat cells, Fetal lung lysate, Human lung or Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab22614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Human HEY1 peptide (ab111660). 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • 组织特异性
    Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • 序列相似性
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all

Anti-HEY1 antibody 图像

  • Lane 1 : Anti-HEY1 antibody (ab22614) at 5 µg/ml
    Lane 2 : Anti-HEY1 antibody (ab22614) at 2.5 µg/ml
    Lane 3 : Anti-HEY1 antibody (ab22614) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human fetal lung cells
    Lane 2 : Cell lysate prepared from human fetal lung cells
    Lane 3 : Cell lysate prepared from human fetal lung cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
  • Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml.  Magnification 400X.

    Alveolar cells are positively labelled (indicated with arrows).

  • ab14404 staining HEY1 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.

    Epithelial cells of renal tubules are positively labelled (indicated with arrows).

Anti-HEY1 antibody (ab22614)参考文献

This product has been referenced in:
  • Suliman MA  et al. Niclosamide inhibits colon cancer progression through downregulation of the Notch pathway and upregulation of the tumor suppressor miR-200 family. Int J Mol Med 38:776-84 (2016). WB ; Human . Read more (PubMed: 27460529) »
  • Deskin B  et al. Requirement of HDAC6 for activation of Notch1 by TGF-ß1. Sci Rep 6:31086 (2016). WB . Read more (PubMed: 27499032) »

See all 24 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Skin fibroblast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Skin fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2017

Abreviews
Application
ChIP
Sample
Mouse Cell lysate - nuclear (SN4741 cells)
Negative control
igG
Specification
SN4741 cells
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Application
Western blot
Sample
Human Purified protein (brain cancer)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain cancer
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Apr 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Placenta)
Specification
Placenta
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 May 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (postnatal day 1 testes)
Specification
postnatal day 1 testes
Fixative
4% PFA
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Qing Wen

Verified customer

提交于 Jan 02 2013

Thank you for contacting us.

I have been in touch with the lab regarding your inquiry. The sample type used in the western blot and IHC images are of normal human lung.The sample type used in the other IHC image is of normal human kidney. ...

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Thank you for calling Abcam earlier today.

I am sorry that you are experiencing issues with ab22614 in IHC-P. As we discussed on the telephone, if you could either send me the Abcam order number or the PO# that was used to purchase the antibod...

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Thank you for taking time to complete our questionnaire and informing us of the problems that your customer has been having with this antibody.

The details provided will enable us to investigate this case and will provide us with vital infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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