概述

  • 产品名称Anti-HEY1抗体
    参阅全部 HEY1 一抗
  • 描述
    兔多克隆抗体to HEY1
  • 经测试应用适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide derived from a region between residues 207-261 of Human HEY1.

  • 阳性对照
    • Jurkat cells, Fetal lung lysate, Human lung or Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab22614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Human HEY1 peptide (ab111660). 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • 组织特异性Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • 序列相似性Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all

Anti-HEY1 antibody 图像

  • Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml.  Magnification 400X.

    Alveolar cells are positively labelled (indicated with arrows).

  • Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.

    Epithelial cells of renal tubules are positively labelled (indicated with arrows).

  • Lane 1 : Anti-HEY1 antibody (ab22614) at 5 µg/ml
    Lane 2 : Anti-HEY1 antibody (ab22614) at 2.5 µg/ml
    Lane 3 : Anti-HEY1 antibody (ab22614) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human fetal lung cells
    Lane 2 : Cell lysate prepared from human fetal lung cells
    Lane 3 : Cell lysate prepared from human fetal lung cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
  • ab14404 staining HEY1 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.

    See Abreview

Anti-HEY1 antibody (ab22614)参考文献

This product has been referenced in:

See all 12 Publications for this product

Product Wall

Abreviews
Application ChIP
Sample Mouse Cell lysate - nuclear (SN4741 cells)
Negative control igG
Specification SN4741 cells
Detection step Semiquantitative PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Application Western blot
Sample Human Purified protein (brain cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification brain cancer
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Apr 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Placenta)
Specification Placenta
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 May 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS1 cell line)
Specification COS1 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (postnatal day 1 testes)
Specification postnatal day 1 testes
Fixative 4% PFA
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Qing Wen

Verified customer

提交于 Jan 02 2013

Thank you for contacting us.

I have been in touch with the lab regarding your inquiry. The sample type used in the western blot and IHC images are of normal human lung.The sample type used in the other IHC image is of normal human kidney. ...

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Thank you for calling Abcam earlier today.

I am sorry that you are experiencing issues with ab22614 in IHC-P. As we discussed on the telephone, if you could either send me the Abcam order number or the PO# that was used to purchase the antibod...

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Thank you for taking time to complete our questionnaire and informing us of the problems that your customer has been having with this antibody.

The details provided will enable us to investigate this case and will provide us with vital infor...

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Thank you for contacting us. When consulting the images provided on the datasheet of ab22614, it seems to that these tissues (lung and kidney) do have a specific staining pattern - and should therefore provide an internal negative control in my opinion...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"