概述

  • 产品名称Anti-HEY1抗体
    参阅全部 HEY1 一抗
  • 描述
    兔多克隆抗体to HEY1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human HEY1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9Y5J3

  • 阳性对照
    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab111723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

靶标

  • 功能Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • 组织特异性Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • 序列相似性Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all

Anti-HEY1 antibody 图像

  • Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-HEY1 antibody (ab111723)参考文献

ab111723 has not yet been referenced specifically in any publications.

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