Anti-Hexokinase Type III抗体(ab91097)

概述

  • 产品名称Anti-Hexokinase Type III抗体
    参阅全部 Hexokinase Type III 一抗
  • 描述
    兔多克隆抗体to Hexokinase Type III
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Hexokinase Type III .

  • 阳性对照
    • This antibody gave a positive signal in both Human liver and spleen tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab91097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

靶标

  • 通路Carbohydrate metabolism; hexose metabolism.
  • 序列相似性Belongs to the hexokinase family.
  • 结构域The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Anti-Hexokinase Type III antibody 图像

  • All lanes : Anti-Hexokinase Type III antibody (ab91097) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 175 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab91097 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91097 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Hexokinase Type III antibody (ab91097)参考文献

ab91097 has not yet been referenced specifically in any publications.

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