The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
组织特异性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
通路Carbohydrate metabolism; hexose metabolism.
序列相似性Belongs to the hexokinase family. Contains 2 hexokinase domains.
结构域The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
细胞定位Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
ab71725 at 1/50 dilution, staining Hexokinase II in human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-Hexokinase II antibody - N-terminal (ab71725)参考文献
has not yet been referenced specifically in any publications.