ab78259 is partially purified rabbit serum. Antibodies reactive with the Type I isozyme were present in the original antiserum; these were removed by affinity chromatography on a column of immobilized Type I isozyme, and the affinity-depleted serum restored to original volume.
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Carbohydrate metabolism; hexose metabolism.
Belongs to the hexokinase family. Contains 2 hexokinase domains.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
Western blot - Anti-Hexokinase II antibody (ab78259)
The Type I isozyme (purified from rat brain) and the rat Type II and Type III isozymes (expressed in yeast) were used for SDS gel electrophoresis and immunoblotting. The top panel shows the gel after staining with Coomassie Blue (5 µg protein in each lane). The bottom three panels show replicate immunoblots probed with a monoclonal to Hexokinase I, ab78259 or a monoclonal to Hexokinase III.
Waskova-Arnostova P et al. Chronic hypoxia enhances expression and activity of mitochondrial creatine kinase and hexokinase in the rat ventricular myocardium. Cell Physiol Biochem33:310-20 (2014).
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