Anti-Hexokinase II抗体(ab76959)

概述

  • 产品名称
    Anti-Hexokinase II抗体
    参阅全部 Hexokinase II 一抗
  • 描述
    小鼠单克隆抗体to Hexokinase II
  • 特异性
    This antibody is likely to cross-react with Hexokinase I (93% identity with the immunogen).
  • 经测试应用
    适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment: IVKEVCTVVA RRAAQLCGAG MAAVVDRIRE NRGLDALKVT VGVDGTLYKL HPHFAKVMHE TVKDLAPKCD VSFLQSEDGS GKGAALITAV ACRIREAGQR , corresponding to amino acids 818-917 of Human Hexokinase IIwith a proprietary tag (MW of the tag alone is 26 KDa).

  • 阳性对照
    • A431 cell lysate
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab104836 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab76959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 组织特异性
    Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • 通路
    Carbohydrate metabolism; hexose metabolism.
  • 序列相似性
    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • 结构域
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 细胞定位
    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

图片

  • Anti-Hexokinase II antibody (ab76959) at 1/1000 dilution + A-431 cell lysate at 25 µg

    Predicted band size : 102 kDa
  • Anti-Hexokinase II antibody (ab76959) at 1/2500 dilution + Immunizing peptide at 0.1 µg

    Predicted band size : 102 kDa
  • Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.
  • Overlay histogram showing K562 cells stained with ab76959 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76959, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献

This product has been referenced in:
  • Xirouchaki CE  et al. Impaired glucose metabolism and exercise capacity with muscle-specific glycogen synthase 1 (gys1) deletion in adult mice. Mol Metab 5:221-32 (2016). Read more (PubMed: 26977394) »
  • Yao M  et al. Dicer mediating the expression of miR-143 and miR-155 regulates hexokinase II associated cellular response to hypoxia. Am J Physiol Lung Cell Mol Physiol 307:L829-37 (2014). Read more (PubMed: 25172909) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Human Cell lysate - whole cell (HCT116, MiaPaca2 and RKO)
Specification
HCT116, MiaPaca2 and RKO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

提交于 Aug 27 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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