概述

  • 产品名称Anti-Hexokinase II抗体
    参阅全部 Hexokinase II 一抗
  • 描述
    兔多克隆抗体to Hexokinase II
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 400~500 of human HK2.

  • 阳性对照
    • A375 cell lysate; Human hepatocarcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab37593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 组织特异性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • 通路Carbohydrate metabolism; hexose metabolism.
  • 序列相似性Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • 结构域The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 细胞定位Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

Anti-Hexokinase II antibody 图像

  • Anti-Hexokinase II antibody (ab37593) at 1/500 dilution + A375 cell lysate

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
    Additional bands at : 30 kDa,40 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab37593, at 1/100 dilution, staining human hepatocarcinoma tissue by immunohistochemistry. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Anti-Hexokinase II antibody (ab37593)参考文献

This product has been referenced in:
  • Fack F  et al. Bevacizumab treatment induces metabolic adaptation toward anaerobic metabolism in glioblastomas. Acta Neuropathol 129:115-31 (2015). WB . Read more (PubMed: 25322816) »
  • Kristian A  et al. Dynamic (18)?F-FDG PET for Assessment of Tumor Physiology in Two Breast Carcinoma Xenografts. Nucl Med Mol Imaging 47:173-80 (2013). WB . Read more (PubMed: 24900104) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 09 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cell line)
Loading amount 30 µg
Specification U2OS cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Renal Cancer)
Specification Renal Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1%
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Verified customer

提交于 Aug 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"