Anti-Hexokinase II抗体(ab227198)
Key features and details
- Rabbit polyclonal to Hexokinase II
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Hexokinase II抗体
参阅全部 Hexokinase II 一抗 -
描述
兔多克隆抗体to Hexokinase II -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig -
免疫原
Recombinant fragment within Human Hexokinase II (internal sequence). The exact sequence is proprietary.
Database link: P52789 -
阳性对照
- WB: Mouse brain tissue lysate; U087 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extract. IHC-P: U-87 xenograft tissue; rat kidney, stomach and heart tissue; mouse muscle tissue. ICC/IF: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227198于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000.
|
|
WB | (1) |
1/5000 - 1/20000. Predicted molecular weight: 102 kDa.
|
说明 |
---|
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/20000. Predicted molecular weight: 102 kDa. |
靶标
-
组织特异性
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. -
通路
Carbohydrate metabolism; hexose metabolism. -
序列相似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
结构域
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
细胞定位
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
-
数据库链接
- Entrez Gene: 3099 Human
- Entrez Gene: 15277 Mouse
- Entrez Gene: 25059 Rat
- Omim: 601125 Human
- SwissProt: P52789 Human
- SwissProt: O08528 Mouse
- SwissProt: P27881 Rat
- Unigene: 406266 Human
see all -
别名
- DKFZp686M1669 antibody
- Hexokinase 2 antibody
- Hexokinase 2 muscle antibody
see all
图片
-
Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution (HRP-conjugated anti-rabbit IgG antibody) + Mouse brain tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 102 kDa7.5% SDS PAGE
-
Paraffin-embedded rat kidney tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
-
Ice cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hexokinase II (green) using ab227198 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
-
All lanes : Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 102 kDa7.5% SDS-PAGE
-
Paraffin-embedded U87 (human) xenograft tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded mouse muscle tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded rat heart tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded rat stomach tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (33)
ab227198 被引用在 33 文献中.
- Wang Y et al. CircPTK2 inhibits cell cisplatin (CDDP) resistance by targeting miR-942/TRIM16 axis in non-small cell lung cancer (NSCLC). Bioengineered 13:3651-3664 (2022). PubMed: 35230201
- Liu X et al. Circ_0078767 Inhibits the Progression of Non-Small-Cell Lung Cancer by Regulating the GPX3 Expression by Adsorbing miR-665. Int J Genomics 2022:6361256 (2022). PubMed: 35340708
- Shen S et al. Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217. J Gene Med 24:e3418 (2022). PubMed: 35357059
- Ding Y et al. Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc. World J Surg Oncol 20:162 (2022). PubMed: 35598017
- Xu M et al. Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway. J Exp Clin Cancer Res 41:253 (2022). PubMed: 35986343