Anti-Hexokinase II抗体[3D3] (ab104836)

概述

  • 产品名称
    Anti-Hexokinase II抗体[3D3]
    参阅全部 Hexokinase II 一抗
  • 描述
    小鼠单克隆抗体[3D3] to Hexokinase II
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Hexokinase II.

  • 阳性对照
    • Jurkat, Hela and HEK293 cell lysates; Human esophagus and lung cancer tissues; K562 cells.

性能

应用

Our Abpromise guarantee covers the use of ab104836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 组织特异性
    Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • 通路
    Carbohydrate metabolism; hexose metabolism.
  • 序列相似性
    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • 结构域
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 细胞定位
    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

图片

  • All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK293 cell lysate


    Predicted band size : 102 kDa
  • Immunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.

    Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

  • Immunofluorescent analysis of paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized MRC5 normal lung fibroblasts labeling Hexokinase with ab104836 at 1/500 dilution,followed by Goat Anti-mouse IgG H&L secondary antibody.

    See Abreview

  • ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
  • ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.

  • ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.

文献

This product has been referenced in:
  • Li Y  et al. Mitochondrial pyruvate carrier function is negatively linked to Warburg phenotype in vitro and malignant features in esophageal squamous cell carcinomas. Oncotarget 8:1058-1073 (2017). WB . Read more (PubMed: 27911865) »
  • Yang HJ  et al. Expression of Glut-1 and HK-II in Pancreatic Cancer and Their Impact on Prognosis and FDG Accumulation. Transl Oncol 9:583-591 (2016). Read more (PubMed: 27916293) »

See all 7 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse prostate (Pten knockout))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization
No
Specification
Mouse prostate (Pten knockout)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Miss. Lucy Penfold

Verified customer

提交于 Nov 09 2016

Application
Western blot
Sample
Baboon Tissue lysate - whole (fetal skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
25 µg
Treatment
115 ug/kg body weight/day letrozole in utero for 100 days
Specification
fetal skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Soon-Ok Kim

Verified customer

提交于 Aug 19 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MRC5 normal lung fibroblasts)
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Specification
MRC5 normal lung fibroblasts
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Feb 27 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100mM sodium Cytrat
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

提交于 Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100mM sodium citrate
Permeabilization
No
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ilan Sela

Verified customer

提交于 May 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
30 µg
Specification
HeLa
Treatment
CoCl2 for 24h
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Hadas Pahima

Verified customer

提交于 Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma neurosphere cells (primary))
Loading amount
30 µg
Specification
Glioblastoma neurosphere cells (primary)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2011

Thank you for your inquiry. We did not test for cross-reactivity with hexokinase I. Please contact us with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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