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Recombinant fragment corresponding to Human Hexokinase II.
Our Abpromise guarantee covers the use of ab104836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 102 kDa.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
Immunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.
Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.
Immunofluorescent analysis of paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized MRC5 normal lung fibroblasts labeling Hexokinase with ab104836 at 1/500 dilution,followed by Goat Anti-mouse IgG H&L secondary antibody.
ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
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