Hexokinase Activity Assay试剂盒(Fluorometric) (ab211103)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 2 µU
概述
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产品名称
Hexokinase Activity Assay试剂盒(Fluorometric)
参阅全部 Hexokinase 试剂盒 -
检测方法
Fluorescent -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Enzyme activity (quantitative) -
灵敏度
2 µU -
种属反应性
与反应: Mammals, Other species -
产品概述
The Hexokinase Activity Assay Kit (Fluorometric) (ab211103) provides a simple, sensitive and quick method for monitoring hexokinase (HK) activity in cells, serum, and animal or plant tissues. In this assay, HK converts glucose into glucose-6-phosphate, which in turn undergoes a series of reactions and reduces the sensitive probe to generate an intense fluorescent product that can be easily detected at Ex/Em = 535/587 nm.
This assay can detect as low as 2 µU of HK activity.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K769 PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric). K769-100 is the same size as the 100 test size of ab211103.
Hexokinase (HK, 6-Phosphate glucose kinase, ATP:D-Hexose 6- Phosphotransferase, ATP-dependent hexokinase, EC 1.1.1.49) is responsible for phosphorylating hexoses (six-carbon sugars) to form hexose phosphate. Hexokinases play an important role in glucose metabolism, as glucose is the most important substrate of hexokinases. Hexokinases are found in many organisms including bacteria, plants and mammals. In mammals, there are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III are referred as “low Km” because of their high affinity for glucose (Km < 1mM), while HK-IV (also known as Glucokinase) has a Km for glucose 100-fold higher and can only phosphorylate glucose when the substrate concentration is high enough.
Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer LX 1 x 25ml ATP II 1 vial Developer IX 1 vial Development Enzyme Mix IX 1 vial HK Positive Control 1 vial HK Substrate 1 x 1ml NADPH Standard 1 vial PicoProbe I 1 x 400µl -
研究领域
图片
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Typical NADPH standard calibration curve.
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Kinetic curves showing Hexokinase activity in positive control (included in kit), lysates from HeLa (0.62 µg), Jurkat (0.65 µg) and MCF-7 cells (0.56 µg), and lysates from orchid leaf (4 µg).
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Hexokinase specific activity in lysates from orchid leaf (4 µg), MCF-7 (0.56 µg), Jurkat (0.65 µg) and HeLa cells (0.62 µg).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab211103 被引用在 1 文献中.
- Valentín-Guillama G et al. HIV-1 Envelope Protein gp120 Promotes Proliferation and the Activation of Glycolysis in Glioma Cell. Cancers (Basel) 10:N/A (2018). PubMed: 30200472