Hexokinase Activity Assay试剂盒(Colorimetric) (ab136957)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Serum, Suspension cells, Tissue, Tissue Extracts
- Sensitivity: 0.1 mU/well
概述
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产品名称
Hexokinase Activity Assay试剂盒(Colorimetric)
参阅全部 Hexokinase 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Tissue, Adherent cells, Suspension cells, Tissue Extracts -
检测类型
Enzyme activity (quantitative) -
灵敏度
< 0.1 mU/well -
检测时间
1h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
Hexokinase Activity Assay Kit (ab136957) uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.
In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.
Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer LX 1 x 25ml Developer Solution III 1 vial Development Enzyme Mix IX 1 vial Hexokinase Positive Control 1 vial HK Coenzyme 1 vial HK Substrate 1 x 1ml NADH Standard I 1 vial -
研究领域
相关产品
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Related Products
图片
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Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.
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Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.
Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).
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In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab136957 被引用在 44 文献中.
- Khurana P et al. Maternal Undernutrition Induces Cell Signalling and Metabolic Dysfunction in Undifferentiated Mouse Embryonic Stem Cells. Stem Cell Rev Rep 19:767-783 (2023). PubMed: 36517693
- Wang J et al. A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability. Cancer Commun (Lond) 42:1008-1027 (2022). PubMed: 35975322
- Ainscough AJ et al. An organ-on-chip model of pulmonary arterial hypertension identifies a BMPR2-SOX17-prostacyclin signalling axis. Commun Biol 5:1192 (2022). PubMed: 36344664
- Haythorne E et al. Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells. Nat Commun 13:6754 (2022). PubMed: 36376280
- Godsman N et al. Metabolic alterations in a rat model of takotsubo syndrome. Cardiovasc Res 118:1932-1946 (2022). PubMed: 33711093