The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
1/250. (see Abreview)
1/200 - 1/250. (see Abreviews)
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
Carbohydrate metabolism; hexose metabolism.
Hexokinase deficiency Neuropathy, hereditary motor and sensory, Russe type
Belongs to the hexokinase family. Contains 2 hexokinase domains.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
All lanes : Anti-Hexokinase 1 antibody (ab65069) at 1/500 dilution
Lane 1 : extracts from HeLa cells Lane 2 : extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 102 kDa Observed band size: 102 kDa Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Hexokinase 1 antibody (ab65069)This image is courtesy of an anonymous Abreview
ab65069 staining Hexokinase 1 (green) in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/250 in PBS-T) for 2 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Hexokinase 1 antibody (ab65069)Image courtesy of an anonymous Abreview.
ab65069 staining Hexokinase 1 in murine bone marrow leukocytes by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with the primary antibody at 1/250 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. DAPI was used for staining nuclei.
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Tegnebratt T et al. Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches. EJNMMI Res4:34 (2014).
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