Anti-Hex抗体- ChIP Grade (ab34222)


  • 产品名称Anti-Hex抗体- ChIP Grade
    参阅全部 Hex 一抗
  • 描述
    兔多克隆抗体to Hex - ChIP Grade
  • 经测试应用适用于: ChIP, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex.

    (Peptide available as ab28465.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Liver Tissue Lysate.



Our Abpromise guarantee covers the use of ab34222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465).
IHC-FoFr 1/100.


  • 功能Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
  • 组织特异性Liver and promyelocytic leukemia cell line HL60.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed during hematopoiesis.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hematopoietically expressed homeobox antibody
    • Hematopoietically-expressed homeobox protein HHEX antibody
    • HEX antibody
    • HHEX antibody
    • HHEX_HUMAN antibody
    • HMPH antibody
    • Homeobox hematopoietically expressed antibody
    • Homeobox protein HEX antibody
    • Homeobox protein PRH antibody
    • HOX11L PEN antibody
    • PRH antibody
    • PRHX antibody
    • Proline rich homeodomain containing transcription factor antibody
    see all

Anti-Hex antibody - ChIP Grade 图像

  • Anti-Hex antibody - ChIP Grade (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

Anti-Hex antibody - ChIP Grade (ab34222)参考文献

This product has been referenced in:
  • Stepanova V  et al. Urokinase-type Plasminogen Activator (uPA) Promotes Angiogenesis by Attenuating Proline-rich Homeodomain Protein (PRH) Transcription Factor Activity and De-repressing Vascular Endothelial Growth Factor (VEGF) Receptor Expression. J Biol Chem 291:15029-45 (2016). Mouse . Read more (PubMed: 27151212) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (16HBE14o- cells and Caco2 cells)
Specification 16HBE14o- cells and Caco2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Sep 12 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34222 and one vial of ab13537.

To check the status of the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step Real-time PCR
Positive control region known to bind this transcription factor
Negative control region known to be devoid of this transcription factor

Abcam user community

Verified customer

提交于 Jan 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Jun 12 2009