Anti-Heterogeneous核Ribonucleoprotein (A1-like)抗体(ab50966)

概述

  • 产品名称
    Anti-Heterogeneous核Ribonucleoprotein (A1-like)抗体
    参阅全部 Heterogeneous Nuclear Ribonucleoprotein (A1-like) 一抗
  • 描述
    兔多克隆抗体to Heterogeneous核Ribonucleoprotein (A1-like)
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: MSKSASPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN, corresponding to N terminal amino acids 1-50 of Human Heterogeneous Nuclear Ribonucleoprotein (A1-like)

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human stomach tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

图片

  • All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab50966, at a concentration of 8µg/ml, staining Heterogeneous Nuclear Ribonucleoprotein (A1-like) in paraffin embedded human stomach tissue sections. The positive cells (indicated by arrows) are epithelial cells of fundic glands.

文献

ab50966 has not yet been referenced specifically in any publications.

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