概述

  • 产品名称Anti-HES4抗体
    参阅全部 HES4 一抗
  • 描述
    兔多克隆抗体to HES4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: AADTPGKPSA SPMAGAPASA SRTPDKPRSA AEHRKSSKPV MEKRRRARIN , corresponding to amino acids 2-51 of Human HES4

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 相关性HES4 may be a transcriptional repressor. It is expressed in stomach, pancreas, ovary, uterus, eye and bone. It has an N-terminal bHLH domain.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • bHLH factor Hes4 antibody
    • Hairy and enhancer of split 4 antibody
    • Transcription factor HES 4 antibody

Anti-HES4 antibody 图像

  • Anti-HES4 antibody (ab66377) at 2 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

Anti-HES4 antibody (ab66377)参考文献

ab66377 has not yet been referenced specifically in any publications.

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