概述

  • 产品名称Anti-Hes1抗体[7H11]
    参阅全部 Hes1 一抗
  • 描述
    小鼠单克隆抗体[7H11] to Hes1
  • 经测试应用适用于: Flow Cyt, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His-tagged recombinant human Hes1 protein purified from E.coli

  • 阳性对照
    • BeWo cell lysate, HT-29 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
IP Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • 结构域Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Anti-Hes1 antibody [7H11] 图像

  • All lanes : Anti-Hes1 antibody [7H11] (ab70576) at 1/500 dilution

    Lane 1 : BeWo cell lysate
    Lane 2 : HT-29 cell lysate


    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing SH-SY5Y cells stained with ab70576 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hes1 antibody [7H11] (ab70576)参考文献

This product has been referenced in:
  • Moutal A  et al. CRMP5 Controls Glioblastoma Cell Proliferation and Survival through Notch-Dependent Signaling. Cancer Res 75:3519-28 (2015). Read more (PubMed: 26122847) »

See 1 Publication for this product

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