Anti-HERV-FRD抗体(ab90733)

概述

  • 产品名称Anti-HERV-FRD抗体
    参阅全部 HERV-FRD 一抗
  • 描述
    兔多克隆抗体to HERV-FRD
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    A synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (

    GSPYSTNCWLCTSSSTETPGTAYPASPREWTSIEAELHISYRWDPNLKGL

    ) of Human HERV-FRD (NP_997465)

  • 阳性对照
    • HT1080 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties. Can make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity.
    SU mediates receptor recognition.
    TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
  • 组织特异性Expressed at higher level in placenta. Expressed at lower level in adrenal, bone marrow, brain, breast, colon, kidney, lung, ovary, peripheral blood lymphocytes, prostate, skin, spleen, testis, thymus, thyroid, trachea.
  • 序列相似性Belongs to the gamma type-C retroviral envelope protein family. HERV class-I FRD env subfamily.
  • 结构域Contains the CKS-17 immunosuppressive domain present in many retroviral envelope proteins. As a synthetic peptide, it inhibits immune function in vitro and in vivo.
  • 翻译后修饰Specific enzymatic cleavages in vivo yield the mature SU and TM proteins.
    The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion.
  • 细胞定位Cell membrane and Virion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EFRD1_HUMAN antibody
    • Endogenous retrovirus group FRD member 1 antibody
    • Envelope polyprotein antibody
    • ERVFRD-1 antibody
    • FLJ41944 antibody
    • FLJ90611 antibody
    • GLLL6191 antibody
    • HERV FRD 6p24.1 provirus ancestral Env polyprotein antibody
    • HERV FRD antibody
    • HERV FRD provirus ancestral Env polyprotein antibody
    • HERV-FRD antibody
    • HERVFRD antibody
    • HERVFRD provirus ancestral Env polyprotein antibody
    • MGC87585 antibody
    • SU antibody
    • Syncytin 2 antibody
    • Syncytin B antibody
    • Syncytin-2 antibody
    • TM antibody
    • Transmembrane protein antibody
    • UNQ6191 antibody
    see all

Anti-HERV-FRD antibody 图像

  • ab90733 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90733 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-HERV-FRD antibody (ab90733) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Gel concentration: 12%

Anti-HERV-FRD antibody (ab90733)参考文献

ab90733 has not yet been referenced specifically in any publications.

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