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Synthetic peptide from the center region of human HERV conjugated to KLH
Our Abpromise guarantee covers the use of ab71115 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).Can be blocked with HERV peptide (ab91514).|
ab71115 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71115 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.