Herring sperm ssDNA (ab46666)

概述

  • 产品名称Herring sperm ssDNA
  • 常规说明This is a ready to use concentrated solution (9-12 mg/ml DNA). However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool ON ICE for at least 5 minutes prior to use. Cooling on ice will reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.


    DNA for hybridization is used to block non-specific binding of probes to membranes. This product is prepared by sonication of purified herring sperm DNA. Sonication shears the large molecular weight DNA to produce fragments in a size range of 587 to 831 base pairs. This range has been shown to be the most effective for hybridizations. The material is monitored during sonication by electrophoresis in order to determine the size range. Once sonication is complete, the material is denatured by boiling. Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. This product can be used as a blocking agent in Northern and Southern blotting as well as Chromatin Immunoprecipitation and other nucleic acid hybridization techniques.

  • 经测试应用适用于: ChIP, In situ hybridization, Blockingmore details

性能

  • 相关性The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process.
  • 细胞定位Nuclear
  • 别名
    • Single stranded deoxyribose nucleic acid
    • Single stranded DNA

应用

Our Abpromise guarantee covers the use of ab46666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent dilution.
In situ hybridization Use at an assay dependent dilution.
Blocking Use at an assay dependent dilution.

实验方案

Herring sperm ssDNA (ab46666)参考文献

This product has been referenced in:
  • Godmann M  et al. Epigenetic mechanisms regulate stem cell expressed genes Pou5f1 and Gfra1 in a male germ cell line. PLoS One 5:e12727 (2010). ChIP . Read more (PubMed: 20856864) »

See 1 Publication for this product

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ab46666 is provided in pure water, it contains no buffer salts or EDTA. I hope this information helps, please do not hesitate to contact me should you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"