Anti-HERC6抗体(ab99949)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HERC6
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 123 - 393 of Human HERC6 (BC035775).

  • 阳性对照
    • WB: HeLa cell lysate. IHC-P: fetal testis tissue.

性能

应用

Our Abpromise guarantee covers the use of ab99949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-P 1/100 - 1/500.

靶标

  • 功能
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • 组织特异性
    Detected in brain, heart, placenta and testis.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.
  • 发展阶段
    Expressed in fetal brain.
  • 细胞定位
    Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 6.3.2. antibody
    • FLJ20637 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 antibody
    • HECT domain and RCC1 like domain containing protein 6 antibody
    • HECT domain and RCC1-like domain 6 antibody
    • HECT domain and RCC1-like domain-containing protein 6 antibody
    • Hect domain and RLD 6 antibody
    • HERC 6 antibody
    • HERC6 antibody
    • HERC6_HUMAN antibody
    • Potential ubiquitin ligase antibody
    • Probable E3 ubiquitin protein ligase HERC6 antibody
    • Probable E3 ubiquitin-protein ligase HERC6 antibody
    see all

图片

  • Anti-HERC6 antibody (ab99949) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
  • ab99949 at 1/100 dilution detecting HERC6 expression in formalin-fixed, paraffin-embedded Human fetal testis tissue.
    Note cytoplasmic staining.

文献

ab99949 has not yet been referenced specifically in any publications.

客户评价及客户问答

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact o...

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Thank you for your enquiry.
I am sorry to hear this vial of ab99949 is giving you trouble. I am curious to know if you have successfully blotted the HeLa cell lysate with any other antibodies.
Thanks in advance for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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