概述

  • 产品名称Anti-HERC4抗体
    参阅全部 HERC4 一抗
  • 描述
    兔多克隆抗体to HERC4
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1007 and 1057 of Human HERC4 (NP_071362.1).

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab85732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
  • 组织特异性Expressed in brain and testis and detected in heart and placenta.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 7 RCC1 repeats.
  • 发展阶段Expressed in fetal brain.
  • 细胞定位Cytoplasm > cytosol. shows a punctate cytoplasmic distribution.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP564G092 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase 4 antibody
    • HECT domain and RCC1 like domain containing protein 4 antibody
    • HECT domain and RCC1-like domain-containing protein 4 antibody
    • Hect domain and RLD 4 antibody
    • HERC 4 antibody
    • HERC4 antibody
    • HERC4_HUMAN antibody
    • KIAA1593 antibody
    • Probable E3 ubiquitin protein ligase HERC4 antibody
    • Probable E3 ubiquitin-protein ligase HERC4 antibody
    see all

Anti-HERC4 antibody 图像

  • All lanes : Anti-HERC4 antibody (ab85732) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 119 kDa
    Observed band size : 119 kDa


    Exposure time : 30 seconds
  • Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-HERC4 antibody (ab85732)参考文献

ab85732 has not yet been referenced specifically in any publications.

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