Anti-HERC4抗体(ab85732)
Key features and details
- Rabbit polyclonal to HERC4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HERC4抗体
参阅全部 HERC4 一抗 -
描述
兔多克隆抗体to HERC4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 1007 and 1057 of Human HERC4 (NP_071362.1).
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阳性对照
- Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 119 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. -
组织特异性
Expressed in brain and testis and detected in heart and placenta. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 7 RCC1 repeats. -
发展阶段
Expressed in fetal brain. -
细胞定位
Cytoplasm > cytosol. shows a punctate cytoplasmic distribution. - Information by UniProt
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数据库链接
- Entrez Gene: 26091 Human
- Entrez Gene: 67345 Mouse
- Entrez Gene: 309758 Rat
- Omim: 609248 Human
- SwissProt: Q5GLZ8 Human
- SwissProt: Q6PAV2 Mouse
- SwissProt: Q5PQN1 Rat
- Unigene: 51891 Human
see all -
别名
- DKFZP564G092 antibody
- HECT and RLD domain containing E3 ubiquitin protein ligase 4 antibody
- HECT domain and RCC1 like domain containing protein 4 antibody
see all
图片
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All lanes : Anti-HERC4 antibody (ab85732) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 119 kDa
Exposure time: 30 seconds -
Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85732 被引用在 1 文献中.
- Xu Y et al. A miRNA-HERC4 pathway promotes breast tumorigenesis by inactivating tumor suppressor LATS1. Protein Cell 10:595-605 (2019). PubMed: 30710319