Anti-HERC2抗体(ab85832)
Key features and details
- Rabbit polyclonal to HERC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HERC2抗体
参阅全部 HERC2 一抗 -
描述
兔多克隆抗体to HERC2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human HERC2 aa 4750-4850.
Database link: O95714 -
阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 527 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 527 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Mental retardation, autosomal recessive 38 -
序列相似性
Contains 1 cytochrome b5 heme-binding domain.
Contains 1 DOC domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 MIB/HERC2 domain.
Contains 21 RCC1 repeats.
Contains 1 ZZ-type zinc finger. -
结构域
The ZZ-type zinc finger mediates binding to SUMO1, and at lowe level SUMO2.
The RCC1 repeats are grouped into three seven-bladed beta-propeller regions. -
翻译后修饰
Phosphorylation at Thr-4827 is required for interaction with RNF8.
Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus. Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 8924 Human
- Entrez Gene: 15204 Mouse
- Omim: 605837 Human
- SwissProt: O95714 Human
- SwissProt: Q4U2R1 Mouse
- Unigene: 434890 Human
- Unigene: 610412 Human
- Unigene: 741019 Human
see all -
别名
- D15F37S1 antibody
- E3 ubiquitin-protein ligase HERC2 antibody
- HECT and RLD domain containing E3 ubiquitin protein ligase 2 antibody
see all
图片
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All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 527 kDa
Observed band size: 527 kDa
Additional bands at: 160 kDa, 260 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunoprecipitation/ Western Blot
For IP: Lane 1: ab85832 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab85832 被引用在 6 文献中.
- Wang LQ et al. LncRNA-Fendrr protects against the ubiquitination and degradation of NLRC4 protein through HERC2 to regulate the pyroptosis of microglia. Mol Med 27:39 (2021). PubMed: 33858325
- Xu S et al. ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability. iScience 24:102534 (2021). PubMed: 34142045
- Harris LD et al. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. J Biol Chem 296:100246 (2021). PubMed: 33853758
- Liu J et al. Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR Protoc 1:100155 (2020). PubMed: 33377049
- Saez I et al. Insights into the ubiquitin-proteasome system of human embryonic stem cells. Sci Rep 8:4092 (2018). PubMed: 29511261
- Moroishi T et al. HERC2 targets the iron regulator FBXL5 for degradation and modulates iron metabolism. J Biol Chem 289:16430-41 (2014). PubMed: 24778179