概述

  • 产品名称Anti-Hepsin抗体
    参阅全部 Hepsin 一抗
  • 描述
    兔多克隆抗体to Hepsin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Dog, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Hepsin.

  • 阳性对照
    • This antibody gave a positive signal in HepG2 whole cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab73133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Plays an essential role in cell growth and maintenance of cell morphology.
  • 组织特异性Present in most tissues, with the highest level in liver.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.4.21. antibody
    • HEPS antibody
    • HEPS_HUMAN antibody
    • Hepsin antibody
    • Hpn antibody
    • Serine protease hepsin antibody
    • Serine protease hepsin catalytic chain antibody
    • Serine protease hepsin non catalytic chain antibody
    • TMPRSS 1 antibody
    • TMPRSS1 antibody
    • Transmembrane protease serine 1 antibody
    see all

Anti-Hepsin antibody 图像

  • Anti-Hepsin antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes
  • ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.

Anti-Hepsin antibody (ab73133)参考文献

ab73133 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab73133.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"