Anti-Hephaestin抗体(ab95137)

概述

  • 产品名称
    Anti-Hephaestin抗体
    参阅全部 Hephaestin 一抗
  • 描述
    兔多克隆抗体to Hephaestin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Hephaestin.

    (Peptide available as ab108244.)

  • 阳性对照
    • This antibody gave a positive signal in WI38 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab95137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

靶标

图片

  • Anti-Hephaestin antibody (ab95137) at 1 µg/ml + WI-38 whole cell lysate (ab3960) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa


    Exposure time : 20 minutes
  • All lanes : Anti-Hephaestin antibody (ab95137) at 1/1000 dilution

    Lane 1 : adult rat cortex
    Lane 2 : neo-natal rat heart
    Lane 3 : 3 month old rat heart
    Lane 4 : rat embryo

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated Anti-rabbit polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa
    Additional bands at : 17 kDa (possible non-specific binding),64 kDa (possible non-specific binding).

    Western blot analysis of rat tissue lysate (20μg/lane) labelling Hephaestin with ab95137 at 1/1000 in 0.1xTBST plus milk for 24 hours at 4ºC. A HRP conjugated Anti-rabbit polyclonal (1/3000) was used as the secondary antibody.

    See Abreview

实验方案

文献

ab95137 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Duodenum)
Specification
Duodenum
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain, heart, embryo)
Loading amount
20 µg
Specification
brain, heart, embryo
Gel Running Conditions
Reduced Denaturing (4-12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册