Anti-Hephaestin抗体(ab95137)

概述

  • 产品名称
    Anti-Hephaestin抗体
    参阅全部 Hephaestin 一抗
  • 描述
    兔多克隆抗体to Hephaestin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Hephaestin.

    (Peptide available as ab108244.)

  • 阳性对照
    • This antibody gave a positive signal in WI38 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab95137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

靶标

Anti-Hephaestin antibody 图像

  • Anti-Hephaestin antibody (ab95137) at 1 µg/ml + WI-38 whole cell lysate (ab3960) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa


    Exposure time : 20 minutes
  • All lanes : Anti-Hephaestin antibody (ab95137) at 1/1000 dilution

    Lane 1 : adult rat cortex
    Lane 2 : neo-natal rat heart
    Lane 3 : 3 month old rat heart
    Lane 4 : rat embryo

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated Anti-rabbit polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa
    Additional bands at : 17 kDa (possible non-specific binding),64 kDa (possible non-specific binding).

    Western blot analysis of rat tissue lysate (20μg/lane) labelling Hephaestin with ab95137 at 1/1000 in 0.1xTBST plus milk for 24 hours at 4ºC. A HRP conjugated Anti-rabbit polyclonal (1/3000) was used as the secondary antibody.

    See Abreview

实验方案

Anti-Hephaestin antibody (ab95137)参考文献

ab95137 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Duodenum)
Specification
Duodenum
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain, heart, embryo)
Loading amount
20 µg
Specification
brain, heart, embryo
Gel Running Conditions
Reduced Denaturing (4-12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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