HepG2细胞质extract裂解液(ab14659)

概述

  • 产品名称HepG2细胞质extract裂解液
    参阅全部 HepG2 细胞裂解液
  • 常规说明Cell line: HepG2 (Human hepatocellular carcinoma).
    We recommend aliquoting the extracts into single use fractions and then storing them.
  • 经测试应用适用于: WB, EMSAmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Hepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

应用

Our Abpromise guarantee covers the use of ab14659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.

HepG2 cytoplasmic extract lysate (ab14659)参考文献

ab14659 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
The exact protocol is proprietary for ab14659, but the cytoplasmic membrane is first lysed in the hypotonic buffer to harverst the nuclei. Then the proteins from the nuclei are extracted with a lysis buffer. The sample...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"