概述

  • 产品名称
    Anti-Hepcidin-25抗体
    参阅全部 Hepcidin-25 一抗
  • 描述
    兔多克隆抗体to Hepcidin-25
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Fr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25.

    (Peptide available as ab31875.)

  • 阳性对照
    • HAMP Human Full-length Recombinant Protein (Tagged) and LPS treated mouse serum.

性能

应用

Our Abpromise guarantee covers the use of ab30760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).

Current batches of ab30760 gave a positive signal in LPS treated serum in WB at a dilution ranging from 1/10 and 1/100. We recommend customers to determine the optimal dilution for their experiments.

IHC-Fr 1/200.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • 组织特异性
    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • 疾病相关
    Hemochromatosis 2B
  • 序列相似性
    Belongs to the hepcidin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 形式
    Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
  • 别名
    • Hamp antibody
    • HEPC antibody
    • HEPC_HUMAN antibody
    • Hepc20 antibody
    • Hepc25 antibody
    • HEPCIDIN antibody
    • Hepcidin 20 antibody
    • Hepcidin 25 antibody
    • Hepcidin antimicrobial peptide antibody
    • Hepcidin-20 antibody
    • Hepcidin25 antibody
    • HFE2 antibody
    • HFE2B antibody
    • LEAP 1 antibody
    • LEAP-1 antibody
    • LEAP1 antibody
    • Liver expressed antimicrobial peptide antibody
    • Liver-expressed antimicrobial peptide 1 antibody
    • PLTR antibody
    • Putative liver tumor regressor antibody
    see all

图片

  • Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.

  • Anti-Hepcidin-25 antibody (ab30760) + LPS treated mouse serum

    Secondary
    Donkey Polyclonal to Anti Rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 3 kDa

    This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)

  • ab30760 staining cultured rat astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab30760 at 1/100 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Hepcidin expression visible perinuclearly,  mainly in the lysosomes.

    See Abreview

  • ab30760 at a 1/200 dilution staining Hepcidin-25 in human artery tissue sections by Immunohistochemistry (frozen sections) incubated for 24 hours at 20°C. Acetone fixed. Blocked with 0.5% BSA for 10 minutes at 20°C. Secondary used at 7µg/ml goat anti-rabbit IgG (H+L) conjugated to biotin. Immunoperoxydase system and AEC were used to develop the color.

    See Abreview

  • Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 3 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutesab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.

文献

This product has been referenced in:
  • de Seny D  et al. Biomarkers of inflammation and innate immunity in atrophic nonunion fracture. J Transl Med 14:258 (2016). IP . Read more (PubMed: 27599571) »
  • Lakhal-Littleton S  et al. An essential cell-autonomous role for hepcidin in cardiac iron homeostasis. Elife 5:N/A (2016). IHC ; Mouse . Read more (PubMed: 27897970) »

See all 14 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver tissue lysate)
Gel Running Conditions
Reduced Denaturing (11% SDS-PAGE)
Loading amount
30 µg
Specification
Liver tissue lysate
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Adult liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0
Permeabilization
No
Specification
Adult liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Ms. Marcia Burch

Verified customer

提交于 Mar 22 2016

Thank you for your enquiry.

I am sorry to confirm this antibody is still not available and for the inconvenience. I contacted the laboratories and they confirmed that another batch is currently in production, but due to difficulties with past ...

Read More
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Mouse Recombinant protein (Mouse cell lysate)
Specification
Mouse cell lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Type
Indirect
Username

Abcam user community

Verified customer

提交于 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Astrocytes culture)
Specification
Astrocytes culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 13 2012

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results and for the delay in getting back to you. The details provided will enable us to investigate this ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (artery)
Specification
artery
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat liver cell lysate)
Loading amount
30 µg
Specification
rat liver cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Nov 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册