抗丙型肝炎病毒NS5a抗体[H26] (ab13833)
Key features and details
- Mouse monoclonal [H26] to Hepatitis C Virus NS5a
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Hepatitis C virus
- Isotype: IgG1
概述
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产品名称
抗丙型肝炎病毒NS5a抗体[H26] -
描述
小鼠单克隆抗体[H26] to丙型肝炎病毒NS5a -
宿主
Mouse -
特异性
Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a. -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
Recombinant full length protein (E. coli).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
H26 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13833于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent dilution.
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WB |
1/1000.
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ICC/IF |
1/50.
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说明 |
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ELISA
Use at an assay dependent dilution. |
WB
1/1000. |
ICC/IF
1/50. |
靶标
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相关性
HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). -
数据库链接
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26664 Hepatitis C virus
- SwissProt: Q5I2N3 Hepatitis C virus
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别名
- HCV antibody
- HCV Non-structural protein 5A antibody
- Non-structural protein 5A antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab13833 被引用在 10 文献中.
- Kim GW & Siddiqui A N6-methyladenosine modification of HCV RNA genome regulates cap-independent IRES-mediated translation via YTHDC2 recognition. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33649237
- Shiode Y et al. Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation. Sci Rep 10:15290 (2020). PubMed: 32943718
- Li J et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
- Shah R et al. Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection. J Vis Exp N/A:N/A (2020). PubMed: 33191939
- Klinker S et al. Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication. J Biol Chem 294:7388-7402 (2019). PubMed: 30862675
- Kuwashiro T et al. Regulation of interferon signaling and HCV-RNA replication by extracellular matrix. Int J Mol Med 42:957-965 (2018). PubMed: 29786754
- Madhvi A et al. A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound. Sci Rep 7:5816 (2017). PubMed: 28725041
- Abdallah C et al. Hepatitis C virus core protein targets 4E-BP1 expression and phosphorylation and potentiates Myc-induced liver carcinogenesis in transgenic mice. Oncotarget 8:56228-56242 (2017). PubMed: 28915586
- Meistermann H et al. A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation. Mol Cell Proteomics 13:3040-8 (2014). PubMed: 25044019
- Scheel TK et al. Analysis of functional differences between hepatitis C virus NS5A of genotypes 1-7 in infectious cell culture systems. PLoS Pathog 8:e1002696 (2012). WB ; Hepatitis C virus . PubMed: 22654662