Anti-Hepatitis C Virus NS4A抗体[S4-13] (Biotin) (ab124526)

概述

  • 产品名称Anti-Hepatitis C Virus NS4A抗体[S4-13] (Biotin)
    参阅全部 Hepatitis C Virus NS4A 一抗
  • 描述
    小鼠单克隆抗体[S4-13] to丙型肝炎病毒NS4A (Biotin)
  • 偶联物Biotin
  • 特异性Specific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes
  • 经测试应用适用于: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Hepatitis C Virus
  • 免疫原

    A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • 表位N terminal region of the NS4 protein (NS4A).
  • 阳性对照
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • 常规说明

    [biotin] / [IgG] = 11.3

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 克隆单克隆
  • 克隆编号S4-13
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
  • 细胞定位Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
  • 数据库链接
    • 别名
      • core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • HCV NS4A antibody
      • Non structural protein 4A antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA-dependent RNA polymerase antibody
      • p7 protein antibody
      • polyprotein antibody
      see all

    Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) 图像

    • Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.

      Predicted band size : 6 kDa
    • ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.

    Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526)参考文献

    ab124526 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antib...

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    Here is more information on how our tetsing discount peogram works.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:...

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    Thank you for your patience.

    I have good news for you: The unconjugated antibody for ab124526 is now in the catalog and available to be ordered. It is ab128685 (Anti-Hepatitis C Virus NS4A antibody [S4-13]):

    http://www.abcam.com/he...

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    Thank you for contacting us.


    I have the following information for you.

    ab13831: This antibody recognizes proteins of genotypes 1a, 1b, 3 and 4, and possibly others, but not 2a. The following antibody recognizes genotype 2a: An...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"