Anti-Hepatitis C Virus NS3抗体[H23] (ab13830)

概述

  • 产品名称
    Anti-Hepatitis C Virus NS3抗体[H23]
    参阅全部 Hepatitis C Virus NS3 一抗
  • 描述
    小鼠单克隆抗体[H23] to丙型肝炎病毒NS3
  • 特异性
    Hepatitis C Virus antigen NS3 helicase domain. Reacts very well with JFH-1 strain (genotype 2a)
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 免疫原

    Recombinant full length protein (E. coli).

  • 表位
    Helicase domain

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    H23
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/1000.
IHC-P 1/50.

Tested using paraffin-embedded liver sections from HCV patients.

靶标

  • 相关性
    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • 细胞定位
    Endoplasmic reticulum membrane
  • 别名
    • Hepatitis C Virus NS3 antibody
    • NS3 antibody
    • NS3P antibody
    • p70 antibody
    • Serine protease/NTPase/helicase antibody
    see all

图片

  • ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.
  • The negative control for above picture with Huh7 cells.
  • Image from Kota S et al., PLoS One. 2012;7(2):e32207. Epub 2012 Feb 28.; Fig 3.; doi:10.1371/journal.pone.0032207; February 28, 2012, PLoS ONE 7(2): e32207.

    Representative Western Blot detecting Hepatitis C Virus NS3 with ab13830.

    SL209-biotin was immobilized on streptavidin agarose beads and incubated with lysates of Huh-7.5 cells infected with HCV J6/JFH-1. Retained proteins were examined by western blot.

文献

This product has been referenced in:
  • Hansen MD  et al. Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M. Proc Natl Acad Sci U S A 114:E3462-E3471 (2017). Read more (PubMed: 28389568) »
  • Chen J  et al. Role of HDAC9-FoxO1 Axis in the Transcriptional Program Associated with Hepatic Gluconeogenesis. Sci Rep 7:6102 (2017). Read more (PubMed: 28733598) »

See all 17 Publications for this product

客户评价及客户问答

Thank you for your message.

Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and fr...

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Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tes...

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Thank you for sending me the image of your blot. So, you have no bands (just some background) in the NS3 recombinant protein lane, and the same pattern of bands in the other lanes, including the negative control. Just to make sure, do you know that you...

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Thank you for your message and I'm sorry to hear that you are experiencing difficulty with ab13830. It would be helpful if you could send me an image of the blot. You mention no signal but then mention non-specific bands. How strong are these bands? Wh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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