Anti-Hepatitis C Virus NS3抗体[806] (ab18671)

概述

  • 产品名称
    Anti-Hepatitis C Virus NS3抗体[806]
    参阅全部 Hepatitis C Virus NS3 一抗
  • 描述
    小鼠单克隆抗体[806] to丙型肝炎病毒NS3
  • 特异性
    ab 18671 reacts with recombinant NS-3 (amino acids 1252-1477), synthetic NS-3 (amino acids 1378-1458), and recombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4a protein (amino acids 1689-1735).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    Purified Recombinant Chimeric HCV Polyprotein (555 a.a)

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    806
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    ab18671 at a concentration of 0.1-1 ug/mL will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein.
  • 靶标

    • 相关性
      HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
    • 细胞定位
      Endoplasmic reticulum membrane
    • 别名
      • Hepatitis C Virus NS3 antibody
      • NS3 antibody
      • NS3P antibody
      • p70 antibody
      • Serine protease/NTPase/helicase antibody
      see all

    实验方案

    文献

    This product has been referenced in:
    • Stindt S  et al. Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family. PLoS One 11:e0148711 (2016). Read more (PubMed: 26886748) »
    • Kong L  et al. HIV infection of hepatocytes results in a modest increase in hepatitis C virus expression in vitro. PLoS One 9:e83728 (2014). WB . Read more (PubMed: 24586227) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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