Anti-Hepatitis C Virus E1抗体[BDI198] (ab21306)


  • 产品名称Anti-Hepatitis C Virus E1抗体[BDI198]
    参阅全部 Hepatitis C Virus E1 一抗
  • 描述
    小鼠单克隆抗体[BDI198] to丙型肝炎病毒E1
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    Hepatitis C Virus.
  • 免疫原

    Recombinant HCV E1 glycoprotein (genotype 1b)

  • 表位Epitope has not been determined.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号BDI198
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab21306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Dilution optimised using Chromogenic detection.


  • 相关性Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
  • 细胞定位Endoplasmic reticulum membrane; Single-pass type I membrane protein
  • 别名
    • E1 protein antibody
    • Envelope glycoprotein E1 antibody
    • Gp32 antibody
    • Gp35 antibody
    • HCV E1 antibody
    • HCV envelope glycoprotein E1 antibody
    • Hepatitis C virus envelope glycoprotein E1 antibody
    see all

Anti-Hepatitis C Virus E1 antibody [BDI198] (ab21306)参考文献

This product has been referenced in:
  • Garrone P  et al. A prime-boost strategy using virus-like particles pseudotyped for HCV proteins triggers broadly neutralizing antibodies in macaques. Sci Transl Med 3:94ra71 (2011). WB . Read more (PubMed: 21813755) »
  • Ivanov AV  et al. Hepatitis C Virus Proteins Activate NRF2/ARE Pathway by Distinct ROS-Dependent and Independent Mechanisms in HUH7 Cells. PLoS One 6:e24957 (2011). WB . Read more (PubMed: 21931870) »

See all 2 Publications for this product

Product Wall

Thank you very much for your interest in ab20852 and ab21306.

To our knowledge, ab20852 and ab21306 havenot been tested in flow cytometry (FC). Therefore, I can offer a discount off a future purchase if you buy ab20852 and ab21306 now, test ...

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Thank you for contacting us.

This anti-HCV E1 antibody is a mouse monoclonal IgG1 which usually comprises 450 amino acids, resulting in a molecular weight of some 150 kDa.

Tomy knowledge, the HCV E1 protein detected by this antibody...

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Thank you for contacting us.

Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience whil...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T kidney and Huh7 liver cell lines)
Loading amount 20 µg
Specification 293T kidney and Huh7 liver cell lines
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA alone, BSA and milk, milk alone at various concentrations (from 1% to 5%) as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Aug 24 2007

Thank you for your enquiry. Ab21306 also reacts with genotype 1b. The immunogen used to generate this antibody was recombinant HCV E1 glycoprotein (genotype 1b) and the epitope has not been determined. The antibody has been tested for application in We...

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