FITC荧光抗丙型肝炎病毒抗体[NS5B-6] (ab122979)
Key features and details
- FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
概述
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产品名称
FITC荧光抗丙型肝炎病毒抗体[NS5B-6]
参阅全部 Hepatitis C Virus 一抗 -
描述
FITC荧光小鼠单克隆抗体[NS5B-6] to丙型肝炎病毒 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes. -
经测试应用
适用于: WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.
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阳性对照
- Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
ab122979 is filter-sterilized. Purity >90% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
NS5B-6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122979于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
细胞定位
Virus surface -
数据库链接
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
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别名
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
图片
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FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 327 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
The NS5b protein is detected as a 58-kDa band. -
ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122979 被引用在 1 文献中.
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245