Anti-Hepatitis C Virus抗体[NS5B-6] (Biotin) (ab122972)

概述

  • 产品名称Anti-Hepatitis C Virus抗体[NS5B-6] (Biotin)
    参阅全部 Hepatitis C Virus 一抗
  • 描述
    小鼠单克隆抗体[NS5B-6] to丙型肝炎病毒(Biotin)
  • 偶联物Biotin
  • 特异性ab122972 recognizes NS5b (also called RNA-directed RNA polymerase).
  • 经测试应用适用于: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Hepatitis C Virus
  • 免疫原

    Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli

  • 阳性对照
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • 常规说明

    Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明filter-sterilized
  • 克隆单克隆
  • 克隆编号NS5B-6
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • 细胞定位Virus surface
  • 数据库链接
    • 别名
      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) 图像

    • Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) + Lysate of chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA

      Predicted band size : 70 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972)参考文献

    This product has been referenced in:
    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »

    See 1 Publication for this product

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