Anti-Hepatitis B Virus Surface Antigen (Ad/Ay)抗体(FITC) (ab20930)

概述

  • 产品名称Anti-Hepatitis B Virus Surface Antigen (Ad/Ay)抗体(FITC)
    参阅全部 Hepatitis B Virus Surface Antigen (Ad/Ay) 一抗
  • 描述
    山羊多克隆抗体to乙型肝炎病毒Surface Antigen (Ad/Ay) (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Purified surface antigen. Monospecific, reacts only with Hepatitis B virus.
  • 经测试应用适用于: ELISAmore details
  • 免疫原

    Hepatitis B surface antigen purified from human serum (mixture of subtypes ad & ay).

  • 常规说明

    Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

性能

应用

Our Abpromise guarantee covers the use of ab20930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

靶标

  • 相关性Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • 细胞定位Virion membrane
  • 别名
    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (FITC) (ab20930)参考文献

ab20930 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral immune cells)
Permeabilization No
Gating Strategy w work on purified cells. pDC were gated on BDCA2+HLADR+ and then
Specification peripheral immune cells
Preparation Cell harvesting/tissue preparation method: isolation of PBMC after ficoll plaque and purification of plasmacytoid dendritic cells
Sample buffer: cells were cultured in RPMI 10% SVF with the viral Ag at 4°C or 37°C and then wash before incubation with this anti-body.
Fixation Paraformaldehyde
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提交于 Oct 27 2016

Thank you for your inquiry.

Please have follow this link for all our Hepatitis B Virus Surface Antigen (Ad/Ay) antibodies that are un-conjugated:

http://www.abcam.com/index.html?pageconfig=searchresults&search=Hepatitis B Virus ...

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This is to confirm that a vial of ab20930 has been added free of charge to Cedarlane's order and will be dispatched today.

Thank you for your enquiry. I can confirm that ab20930 Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody is a goat polyclonal antibody. Is is very unusual to have isotype controls for polyclonal antibodies, becuase they contain a mixture of i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"