抗乙型肝炎病毒Core Antigen抗体[C1] (ab8637)

概述

  • 产品名称
    抗乙型肝炎病毒Core Antigen抗体[C1]
    参阅全部 Hepatitis B Virus Core Antigen 一抗
  • 描述
    小鼠单克隆抗体[C1] to乙型肝炎病毒Core Antigen
  • 宿主
    Mouse
  • 特异性
    This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
  • 经测试应用
    适用于: ELISA, IHC-Fr, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Hepatitis B virus
  • 免疫原

    Purified Hepatitis B Core Antigen

  • 表位
    Around aa positions 70-80, major, or "c1" determinant

性能

应用

Our Abpromise guarantee covers the use of ab8637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
WB 1/1000. We recommend boiling the sample for 10 minutes before loading.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • 细胞定位
    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • 数据库链接
    • 别名
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    图片

    • Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

      Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

    文献

    This product has been referenced in:
    • Liu B  et al. Hepatitis B virus X protein inhibits autophagic degradation by impairing lysosomal maturation. Autophagy 10:416-30 (2014). Read more (PubMed: 24401568) »
    • Hasmoni SS  et al. Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage. J Gen Appl Microbiol 51:125-31 (2005). Read more (PubMed: 15942873) »

    See all 4 Publications for this product

    客户评价及客户问答

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7 Human Hepatoma cells)
    Specification
    Huh7 Human Hepatoma cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Saponin
    Username

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    Verified customer

    提交于 Nov 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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