Anti-Hepatitis B Virus Core Antigen抗体[14E11] (ab8638)

概述

  • 产品名称Anti-Hepatitis B Virus Core Antigen抗体[14E11]
    参阅全部 Hepatitis B Virus Core Antigen 一抗
  • 描述
    小鼠单克隆抗体[14E11] to乙型肝炎病毒Core Antigen
  • 经测试应用适用于: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Hepatitis B virus
  • 免疫原

    Purified Denatured Hepatitis B Core Antigen

  • 表位135-141aa (PNAPILS)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Bioreactor harvest, serum-free medium, no protein added
  • Concentration information loading...
  • 纯化说明Bioreactor harvest: Antibodies generated as bioreactor harvest produce less background as they are less contaminated with other proteins compared to ascites.
  • 克隆单克隆
  • 克隆编号14E11
  • 骨髓瘤Sp2/0
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/1000.

靶标

  • 相关性Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • 细胞定位Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • 数据库链接
    • 别名
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)参考文献

    This product has been referenced in:
    • Gutelius D  et al. Characterization of the pleiotropic effects of the genotype g-specific 36-nucleotide insertion in the context of other hepatitis B virus genotypes. J Virol 85:13278-89 (2011). Read more (PubMed: 21994450) »
    • Martínez CA  et al. Exploring different strategies to express Dengue virus envelope protein in a plant system. Biotechnol Lett 32:867-75 (2010). WB . Read more (PubMed: 20213522) »

    See all 4 Publications for this product

    Product Wall

    We unfortunately do not sell starter packs so I am sorry we cannot send the same. These products are stored in standard amount and the information is available on the datasheets.

    Thank for your inquiry. I would like to reassure you that the antibody is the same as previously. There is no batch to batch variability of the immunogen sequence in monoclonal antibodies. This a monoclonal antibody was known to bind to the HBV...

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    1. The epitope is 135-141 (PNAPILS) 2. This depends on how much virus or core antigen is in the cells. You can load different amounts in parallel. For example 5ug (microgram) and 20ug. 3. Example version of Laemmli 2X buffer 4% SDS 10% 2-me...

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    Thank you for your enquiry. Further to discussions with the lab I have determined that the approach that you are using (n)on-reducing gel at 12%) is not the best system to isolate monomeric units of HBcAg core particles. To dissolve HBcAg core part...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am surprised by the size of the band that you have been detecting. I am in correspondence with the source of this antibody to determine the ...

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    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recogni...

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    Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being c...

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    I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H...

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    Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in...

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    Thank you for your reply. The only data we have regarding this is that it is an ayw variant as described in: Bichko et. al. Subtype ayw variant of hepatitis B virus. DNA primary structure analysis. FEBS Lett. 1985 Jun 3;185(1):208-12.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"