概述
- 产品名称Anti-Heparanase 1抗体
参阅全部 Heparanase 1 一抗 - 描述兔多克隆抗体to Heparanase 1
- 特异性This antibody reacts specifically with human 62 kDa HPSE protein, Heparanase 50 kDa subunit and Heparanase 8 kDa subunit.
- 经测试应用适用于: WBmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow
- 免疫原
Synthetic peptide derived from N terminal region of human Heparanase 1.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: None
Constituents: Whole serum - 纯度Whole antiserum
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab59787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 57 kDa (predicted molecular weight: 62 kDa). |
靶标
- 功能Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
- 组织特异性Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
- 序列相似性Belongs to the glycosyl hydrolase 79 family.
- 翻译后修饰Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility. - 细胞定位Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
- Information by UniProt
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数据库链接
- Entrez Gene: 281230 Cow
- Entrez Gene: 10855 Human
- Entrez Gene: 15442 Mouse
- Entrez Gene: 64537 Rat
- Omim: 604724 Human
- SwissProt: Q9Y251 Human
- SwissProt: Q6YGZ1 Mouse
- SwissProt: Q71RP1 Rat
see all -
别名
- Endo glucoronidase antibody
- Endo-glucoronidase antibody
- HEP antibody
see all
Anti-Heparanase 1 antibody 图像
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Western blot - Heparanase 1 antibody (ab59787)All lanes : Anti-Heparanase 1 antibody (ab59787) at 1/1000 dilution
Lane 1 :Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 62 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?) -
Western blot - Heparanase 1 antibody (ab59787)Anti-Heparanase 1 antibody (ab59787) + Human epidermis
Predicted band size : 62 kDa
Observed band size : 8,50 kDa (why is the actual band size different from the predicted?)
Anti-Heparanase 1 antibody (ab59787)参考文献
ab59787 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"