Anti-Heparanase 1抗体(ab59787)

概述

  • 产品名称Anti-Heparanase 1抗体
    参阅全部 Heparanase 1 一抗
  • 描述
    兔多克隆抗体to Heparanase 1
  • 特异性This antibody reacts specifically with human 62 kDa HPSE protein, Heparanase 50 kDa subunit and Heparanase 8 kDa subunit.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide derived from N terminal region of human Heparanase 1.

性能

应用

Our Abpromise guarantee covers the use of ab59787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 57 kDa (predicted molecular weight: 62 kDa).

靶标

  • 功能Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
  • 组织特异性Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
  • 序列相似性Belongs to the glycosyl hydrolase 79 family.
  • 翻译后修饰Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
    N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
  • 细胞定位Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endo glucoronidase antibody
    • Endo-glucoronidase antibody
    • HEP antibody
    • Heparanase 50 kDa subunit antibody
    • Heparanase antibody
    • Heparanase-1 antibody
    • Heparanase1 antibody
    • Hpa 1 antibody
    • HPA antibody
    • Hpa1 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • HPSE 1 antibody
    • HPSE antibody
    • HPSE_HUMAN antibody
    • HPSE1 antibody
    • HSE 1 antibody
    • HSE1 antibody
    see all

Anti-Heparanase 1 antibody 图像

  • All lanes : Anti-Heparanase 1 antibody (ab59787) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 62 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • Anti-Heparanase 1 antibody (ab59787) + Human epidermis

    Predicted band size : 62 kDa
    Observed band size : 8,50 kDa (why is the actual band size different from the predicted?)

Anti-Heparanase 1 antibody (ab59787)参考文献

ab59787 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Rat Cell (Sciatic nerve)
Permeabilization No
Specification Sciatic nerve
Fixative Paraformaldehyde
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提交于 Oct 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"