Anti-Heparanase 1抗体(ab228660)
Key features and details
- Rabbit polyclonal to Heparanase 1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Heparanase 1抗体
参阅全部 Heparanase 1 一抗 -
描述
兔多克隆抗体to Heparanase 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Human -
免疫原
Synthetic peptide within Human Heparanase 1 aa 140-190 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (50kDa subunit).
Database link: Q9Y251 -
阳性对照
- WB: Mouse lymph node lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100 - 1/1000. Predicted molecular weight: 61 kDa.
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说明 |
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WB
1/100 - 1/1000. Predicted molecular weight: 61 kDa. |
靶标
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功能
Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies. -
组织特异性
Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. -
序列相似性
Belongs to the glycosyl hydrolase 79 family. -
翻译后修饰
Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility. -
细胞定位
Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium. - Information by UniProt
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数据库链接
- Entrez Gene: 10855 Human
- Entrez Gene: 15442 Mouse
- Omim: 604724 Human
- SwissProt: Q9Y251 Human
- SwissProt: Q6YGZ1 Mouse
- Unigene: 44227 Human
- Unigene: 265786 Mouse
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别名
- Endo glucoronidase antibody
- Endo-glucoronidase antibody
- HEP antibody
see all
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab228660 尚未被引用在任何文献中。