Anti-Heparan Sulfate Proteoglycan 2抗体[A74] (ab23418)

概述

  • 产品名称Anti-Heparan Sulfate Proteoglycan 2抗体[A74]
    参阅全部 Heparan Sulfate Proteoglycan 2 一抗
  • 描述
    小鼠单克隆抗体[A74] to Heparan Sulfate Proteoglycan 2
  • 特异性ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • 经测试应用适用于: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Corneal endothelial cells (Cow).

  • 表位Epitope is located in domain V of Perlecan.

性能

应用

Our Abpromise guarantee covers the use of ab23418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 22278369
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 469 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.
ELISA 1/2000.
AP Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.

靶标

  • 功能Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • 组织特异性Found in the basement membranes.
  • 疾病相关Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • 序列相似性Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • 翻译后修饰Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all

Anti-Heparan Sulfate Proteoglycan 2 antibody [A74] 图像

  • ab23418 staining Heparan Sulfate Proteoglycan 2 in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2 with ab23418.

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.

    See Abreview

Anti-Heparan Sulfate Proteoglycan 2 antibody [A74] (ab23418)参考文献

This product has been referenced in:
  • Su L  et al. Identification of novel biomarkers for sepsis prognosis via urinary proteomic analysis using iTRAQ labeling and 2D-LC-MS/MS. PLoS One 8:e54237 (2013). WB ; Human . Read more (PubMed: 23372690) »
  • Mustafa DA  et al. A proteome comparison between physiological angiogenesis and angiogenesis in glioblastoma. Mol Cell Proteomics 11:M111.008466 (2012). IHC-P ; Human . Read more (PubMed: 22278369) »

See all 5 Publications for this product

Product Wall

Thank you once again for your enquiries. I was in touch with our source for these antibodies again today, and I am pleased I now have the information for you.   1. Can ab26265 and ab23418 be used to detect for all kind of Heparan sulfate or are they...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human decelluarized adipose matrix)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer pH 6.0 for 40 minutes at 97.0 ±1 °C
Permeabilization No
Specification human decelluarized adipose matrix
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
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提交于 Jul 29 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample Human Tissue sections (human whole fat tissue)
Specification human whole fat tissue
Permeabilization No
Fixative Formaldehyde
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提交于 Mar 05 2015

The epitope of ab23418 is located in domain V of Perlecan, which is from C-terminal amino acids 3663-4391 according to the uniprot file below:

http://www.uniprot.org/uniprot/P98160

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human fibroblast)
Specification Human fibroblast
Fixative Methanol
Permeabilization Yes - triton X-100 1%
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jun 28 2012

Thank you for calling Abcam earlier today.
I am sorry that you experienced issues with ab23418 in western blot. As we discussed I am sending you ab82346 as a free of charge replacement. Your new order number is *********
.
Please let me ...

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Thank you very much for contacting us and for your patience. I apologise for the delay.

We have contacted the originator of the Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]ab23418and enquired about epitope details. Unfortunately, there ...

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Code de remise pour ab86750 : ******
Code de remise pour ab23418 : ******
Date d’expiration pour ces deux codes : JJ MM AAAA
Je suis ravi que vous souhaitiez participer à notre offre et tester ab86750 et ab23418 en ICC/IF. Les codes de r...

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Thank you for your inquiry. Unfortunately we have no information on any aa sequences for this antibody other than it is corneal endothelial cells from cow. Below is listed the following prices: Please let me know if I can be of furt...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 50 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10%SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 08 2010

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