RabMAb

Anti-Hemopexin抗体[EPR5610] (ab124935)

概述

性能

应用

Our Abpromise guarantee covers the use of ab124935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

靶标

  • 功能Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻译后修饰N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 1B glycoprotein antibody
    • Beta-1B-glycoprotein antibody
    • FLJ56652 antibody
    • HEMO antibody
    • HEMO_HUMAN antibody
    • Hemopexin antibody
    • Hpx antibody
    • HX antibody
    see all

Anti-Hemopexin antibody [EPR5610] 图像

  • ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • All lanes : Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution

    Lane 1 : Fetal liver lysate
    Lane 2 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size : 52 kDa
    Observed band size : 70-75 kDa (why is the actual band size different from the predicted?)
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Hemopexin antibody [EPR5610] (ab124935)参考文献

ab124935 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human liver)
Specification human liver
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Mar 12 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Tissue lysate - whole (hu liver, hu colon, hu colon carcinoma & bovine li)
Specification hu liver, hu colon, hu colon carcinoma & bovine li
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Steffen Rickelt

Verified customer

提交于 Jan 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"