概述

  • 产品名称
    Anti-Hemopexin抗体
    参阅全部 Hemopexin 一抗
  • 描述
    兔多克隆抗体to Hemopexin
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the Center region of Human Hemopexin.

  • 阳性对照
    • Mouse liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab90947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 52 kDa.
ELISA 1/1000.

靶标

  • 功能
    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 序列相似性
    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻译后修饰
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta 1B glycoprotein antibody
    • Beta-1B-glycoprotein antibody
    • FLJ56652 antibody
    • HEMO antibody
    • HEMO_HUMAN antibody
    • Hemopexin antibody
    • Hpx antibody
    • HX antibody
    see all

Anti-Hemopexin antibody 图像

  • Anti-Hemopexin antibody (ab90947) at 1/1000 dilution + Mouse liver tissue lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 52 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

实验方案

Anti-Hemopexin antibody (ab90947)参考文献

This product has been referenced in:
  • Zauberman A  et al. Host Iron Nutritional Immunity Induced by a Live Yersinia pestis Vaccine Strain Is Associated with Immediate Protection against Plague. Front Cell Infect Microbiol 7:277 (2017). Read more (PubMed: 28680860) »
  • Nath KA  et al. Age sensitizes the kidney to heme protein-induced acute kidney injury. Am J Physiol Renal Physiol 304:F317-25 (2013). Mouse . Read more (PubMed: 23195679) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization
Yes - 0.1% TRITON
Specification
Mouse Kidney
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2%
Fixative
Paraformaldehyde
Username

Dra. Marta Garcia Ramirez

Verified customer

提交于 Jan 22 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HepG2)
Specification
HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human liver)
Specification
human liver
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2013


I would suggest running the gel under reducing conditions as the protein has 6 disulfide bonds which can change the protein's running behaviour. This will linearize the protein.

Also the protein has multiple glycosylation sites which will...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
50 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 25 2012

Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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