Anti-Hemoglobin subunit alpha抗体(ab82871)

概述

  • 产品名称
    Anti-Hemoglobin subunit alpha抗体
    参阅全部 Hemoglobin subunit alpha 一抗
  • 描述
    兔多克隆抗体to Hemoglobin subunit alpha
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Chimpanzee, Monkey, Baboon, Macaque monkey, Rhesus monkey, Gorilla, Dolphin, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 100 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab93083, ab93083)

  • 阳性对照
    • This antibody gave a positive signal in human bone marrow and spleen tissue lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • 组织特异性
    Red blood cells.
  • 疾病相关
    Heinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • 序列相似性
    Belongs to the globin family.
  • 翻译后修饰
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 globin antibody
    • Alpha globin antibody
    • Alpha one globin antibody
    • Alpha-globin antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • Hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin subunit alpha antibody
    • MGC126895 antibody
    • MGC126897 antibody
    see all

Anti-Hemoglobin subunit alpha antibody 图像

  • All lanes : Anti-Hemoglobin subunit alpha antibody (ab82871) at 1/250 dilution

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of Hemoglobin A1c staining in human bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab17976 staining Hemoglobin A1c in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody

    See Abreview

Anti-Hemoglobin subunit alpha antibody (ab82871)参考文献

ab82871 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册