HRP Anti-Hemoglobin抗体(ab19362)
Key features and details
- HRP Goat polyclonal to Hemoglobin
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
概述
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产品名称
HRP Anti-Hemoglobin抗体
参阅全部 Hemoglobin 一抗 -
描述
HRP山羊多克隆抗体to Hemoglobin -
宿主
Goat -
偶联物
HRP -
特异性
The antibody has been tested in ELISA and IEP with a Human Hemaglobin Calibrator/Standard but has yet to be tested against endogenous protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Dog, Chimpanzee, Rhesus monkey -
免疫原
Full length protein corresponding to Human Hemoglobin conjugated to bovine serum albumin. Goats were immunized with purified human hemoglobin A1 of adult red blood cell origin.
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阳性对照
- recombinant human hemoglobin
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常规说明
Molar enzyme/antibody protein ratio is 4:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/30000. Predicted molecular weight: 16 kDa.
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说明 |
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WB
1/1000 - 1/30000. Predicted molecular weight: 16 kDa. |
靶标
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功能
Involved in oxygen transport from the lung to the various peripheral tissues. -
组织特异性
Red blood cells. -
疾病相关
Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
序列相似性
Belongs to the globin family. -
翻译后修饰
The initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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数据库链接
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Entrez Gene: 3043 Human
- Entrez Gene: 3047 Human
- Entrez Gene: 693930 Rhesus monkey
- Omim: 141800 Human
- Omim: 141900 Human
- Omim: 142200 Human
see all -
别名
- 3-prime alpha-globin gene antibody
- A gamma globin antibody
- Alpha 1 globin antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab19362 被引用在 4 文献中.
- Posta N et al. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction. Lab Invest 100:986-1002 (2020). PubMed: 32054994
- Nyakundi BB et al. Oxidized hemoglobin forms contribute to NLRP3 inflammasome-driven IL-1ß production upon intravascular hemolysis. Biochim Biophys Acta Mol Basis Dis 1865:464-475 (2019). PubMed: 30389578
- Potor L et al. Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion. Oxid Med Cell Longev 2018:3812568 (2018). PubMed: 29560080
- Gáll T et al. Heme Induces Endoplasmic Reticulum Stress (HIER Stress) in Human Aortic Smooth Muscle Cells. Front Physiol 9:1595 (2018). PubMed: 30515102