Anti-Hemoglobin抗体[11-201.11] (ab41020)

概述

  • 产品名称Anti-Hemoglobin抗体[11-201.11]
    参阅全部 Hemoglobin 一抗
  • 描述
    小鼠单克隆抗体[11-201.11] to Hemoglobin
  • 特异性ab41020 is specific to human hemoglobin.
  • 经测试应用适用于: ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Sheep, Horse, Chicken, Cow, Pig
  • 免疫原

    Purified human hemoglobin.

性能

应用

Our Abpromise guarantee covers the use of ab41020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/409600.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in oxygen transport from the lung to the various peripheral tissues.
  • 组织特异性Red blood cells.
  • 疾病相关Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • 序列相似性Belongs to the globin family.
  • 翻译后修饰The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-prime alpha-globin gene antibody
    • Alpha 2 globin chain antibody
    • Alpha globin antibody
    • alpha one globin antibody
    • alpha-1 globin antibody
    • Alpha-globin antibody
    • Beta globin antibody
    • CD113t C antibody
    • CD31 antibody
    • Delta globin antibody
    • Erythremia, beta-globin type, included antibody
    • Gamma 1 globin antibody
    • Hb F Agamma antibody
    • HBA 1 antibody
    • HBA 2 antibody
    • HBA antibody
    • HBA T3 antibody
    • HBA-T2 antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • HBB antibody
    • Hbb-y antibody
    • HBD antibody
    • Hbe1 antibody
    • HBG 1 antibody
    • HBG antibody
    • HBG1 antibody
    • HBGA antibody
    • HBGR antibody
    • HBH antibody
    • Hemoglobin alpha 1 antibody
    • hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin alpha locus antibody
    • Hemoglobin alpha locus 1 antibody
    • hemoglobin alpha-1 chain antibody
    • Hemoglobin beta antibody
    • Hemoglobin beta chain antibody
    • Hemoglobin beta chain complex antibody
    • Hemoglobin beta locus antibody
    • Hemoglobin gamma 1 chain antibody
    • Hemoglobin gamma A antibody
    • Hemoglobin gamma A chain antibody
    • Hemoglobin gamma antibody
    • Hemoglobin subunit alpha antibody
    • Hemoglobin subunit beta antibody
    • Hemoglobin subunit gamma 1 antibody
    • hemoglobin, gamma, regulator of antibody
    • Hemoglobin--gamma locus, 136 alanaine antibody
    • HSGGL1 antibody
    • LVV-hemorphin-7 antibody
    • Methemoglobinemia, beta-globin type, included antibody
    • MGC126895 antibody
    • MGC126897 antibody
    • Minor alpha-globin locus antibody
    • PRO2979 antibody
    see all

Anti-Hemoglobin antibody [11-201.11] 图像

  • Overlay histogram showing K562 cells stained with ab41020 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41020, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Hemoglobin antibody [11-201.11] (ab41020)参考文献

ab41020 has not yet been referenced specifically in any publications.

Product Wall

These two products will be suitable standards:

Hemoglobin - ab77858 (purified from Human erythrocytes)
Haptoglobin - ab77872 (purified from Human plasma)

Please contact us if you have additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"