概述

  • 产品名称Anti-Hemoglobin A1c抗体
    参阅全部 Hemoglobin A1c 一抗
  • 描述
    兔多克隆抗体to Hemoglobin A1c
  • 特异性ab31152 is specific to Hemoglobin A1c.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Hemoglobin A1c.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.14M Sodium chloride, 10mM PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified IgG is obtained by synthetic peptide coupling affinity chromatography. Purity was determined by SDS PAGE (blue stain), showing that the IgG constitute was 90% of total protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/2000.
    Immunochromatography: Use at an assay dependent dilution.
    Immunoturbidimetry: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in oxygen transport from the lung to the various peripheral tissues.
      LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
      Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
    • 组织特异性Red blood cells.
    • 疾病相关Heinz body anemias
      Beta-thalassemia
      Sickle cell anemia
      Beta-thalassemia, dominant, inclusion body type
    • 序列相似性Belongs to the globin family.
    • 翻译后修饰Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
      S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
      Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta-globin antibody
      • HBB antibody
      • HBB_HUMAN antibody
      • Hemoglobin beta chain antibody
      • Spinorphin antibody
      see all

    Anti-Hemoglobin A1c antibody 图像

    • All lanes : Anti-Hemoglobin A1c antibody (ab31152) at 1 µg/ml

      Lane 1 : Human thymus tissue lysate - total protein (ab30146)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 15 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    Anti-Hemoglobin A1c antibody (ab31152)参考文献

    This product has been referenced in:
    • Chen SP  et al. Gold nanoparticles-coated magnetic microspheres as affinity matrix for detection of hemoglobin A1c in blood by microfluidic immunoassay. Biosens Bioelectron 26:4779-84 (2011). Immunoelectrophoresis ; Human . Read more (PubMed: 21719268) »

    See 1 Publication for this product

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    Thank you for contacting us.

    We have not used ab31152 and ab82273 product together in ELISA. However based on the nature of protein and immunogen of antibody it seems that both of these product can work together. I am sorry we will not be abl...

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    Hemoglobin A1c is formed by the non-enzymatic attachment of glucose to the N-terminal valine of the β-chain of hemoglobin. ab31152 antibody is specifically designed to detect hemoglobin A1c. Hemoblobin is a heterodimer composed of an alpha and beta...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"